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polyvinylidine fluoride membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad polyvinylidine fluoride membrane
    Polyvinylidine Fluoride Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidine+fluoride+membrane/Trans-Blot+SD/pmc11719836-187-24-32
    Average 99 stars, based on 3734 article reviews
    polyvinylidine fluoride membrane - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Influence of nutrient ingestion on amino acid transporters and protein synthesis in human skeletal muscle after sprint exercise.
    Article Snippet: Håkan C. Rundqvist, Mona Esbjörnsson, Olav Rooyackers, Ted Österlund, Marcus Moberg, William Apro, Eva Blomstrand, and Eva Jansson Division of Clinical Physiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden; Division of Anesthesiology and Intensive Care, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden; Åstrand Laboratory, Swedish School of Sport and Health Science, Stockholm, Sweden; and Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden

    Article Title: Impact of Delta SARS-CoV-2 Infection on Glucose Metabolism: Insights on Host Metabolism and Virus Crosstalk in a Feline Model
    Article Snippet: Briefly, tissues were homogenized in 1:500 dilution of radioimmunoprecipitation assay (RIPA) buffer (ThermoFisher, product #89901, Waltham, MA, USA) with protease inhibitor cocktail (Sigma Aldrich, product #P8340, St. Louis, MO, USA), and quantified via bicinchoninic assay according to manufacturer’s instructions (ThermoFisher, product #23227, Waltham, MA, USA). .. Equal amounts of protein (30–70 μg) were loaded and transferred to a polyvinylidine fluoride membrane (BioRad, Hercules, CA, USA). ..

    Article Title: Purification of micrococcal nuclease for use in ribosomal profiling of high-salinity extremophiles
    Article Snippet: .. After electrophoresis, the gel was equilibrated in Bjerrum Schafer-Nielsen transfer buffer (48 mM tris, 39 mM glycine, 20% methanol), and proteins were transferred to polyvinylidine fluoride membrane using Trans-Blot SD transfer cell (Biorad). .. After transfer, the membrane was blocked in 5% milk in 1×PBS, 0.05% Tween-20 (PBST) for 1 h followed by incubation with 1:2000 diluted 6×-His tag mAb (produced in mouse; Invitrogen; #MA1-21315) at 4 °C, overnight.

    Article Title: Endurance Exercise Enhances the Effect of Strength Training on Muscle Fiber Size and Protein Expression of Akt and mTOR
    Article Snippet: .. The gel was then incubated in transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% methanol) at 4°C for 30 min before electrophoretic transfer of the proteins to a polyvinylidine fluoride membrane (Bio-Rad, Laboratories, Richmond, CA) for 3 h on ice in a 4°C cold room. .. The membrane was subsequently blocked at room temperature for 1 h in TBS (Tris buffered saline; 10 mM Tris pH 7.6, 100 mM NaCl) containing 5% non-fat dry milk and then incubated overnight at 4°C with commercially available primary antibodies diluted in TBS containing 0.1% Tween 20 (TBST) with 2.5% non-fat dry milk: GAPDH (1:5,000), Akt (1:1,000), mTOR (1:1,000), S6K1 (1:1,000) purchased from Cell Signaling Technology (Beverly, MA); α-tubulin (1:5,000) from Sigma-Aldrich (St. Louis, MO); MAFbx (1:1,000) from Abcam (Cambridge, UK) and MuRF1 (1:1,000) from Santa Cruz Biotechnology (Santa Cruz, CA).

    Article Title: Overexpression of Diacylglycerol Kinase η Enhances G α q -Coupled G Protein–Coupled Receptor Signaling
    Article Snippet: A 15- μ l portion of each lysate used in the in vitro kinase assay was mixed with an appropriate amount of 4× Laemmli sample buffer—125 mM Tris-HCl, 40% (v/v) glycerol, 2% (v/v) SDS, 10% (v/v) β -mercaptoethanol (Bio-Rad, Hercules, CA), 0.02% (w/v) bromophenol blue—and separated by SDS-PAGE on a 4% to 15% gradient gel (Bio-Rad) at 100 V for 1 hour. .. Proteins were transferred to a polyvinylidine fluoride membrane (Bio-Rad) at 100 V for 70 minutes on ice and blocked with 5% nonfat milk (Bio-Rad) in Tris-buffered saline/Tween 20 (TBST; 100 mM Tris-HCl pH 7.5, 165 mM NaCl, 0.1% Tween 20) for 1 hour at room temperature. .. Blots were then incubated with rabbit anti-RFP primary antibody (Invitrogen) in 5% BSA (Sigma-Aldrich) in TBST overnight at 4°C.

    Article Title: Transglutaminase 2 exacerbates α-synuclein toxicity in mice and yeast
    Article Snippet: .. Samples were electrophoresed on a NuPage 4–12% Bis-Tris Gel (Invitrogen), and separated proteins were transferred to a polyvinylidine fluoride membrane (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Striatal ΔFosB gene suppression inhibits the development of abnormal involuntary movements induced by L-Dopa in rats
    Article Snippet: Protein concentrations were determined using the BCA assay (Pierce). .. Lysates (20 μg) were mixed with lithium dodecyl sulfate-sample loading buffer (Life Technologies, Grand Island, NY), separated on a NuPage 4–20% (GenScript, Piscataway, NJ), and transferred onto a polyvinylidine fluoride membrane (Bio-Rad, Hercules, CA). .. Blots were blocked in 5% BSA (Sigma-Aldrich, St. Louis, MO) in Tris-buffered saline and 0.1% Tween-20 before probing with antibodies.

    Electrophoresis:

    Article Title: Purification of micrococcal nuclease for use in ribosomal profiling of high-salinity extremophiles
    Article Snippet: .. After electrophoresis, the gel was equilibrated in Bjerrum Schafer-Nielsen transfer buffer (48 mM tris, 39 mM glycine, 20% methanol), and proteins were transferred to polyvinylidine fluoride membrane using Trans-Blot SD transfer cell (Biorad). .. After transfer, the membrane was blocked in 5% milk in 1×PBS, 0.05% Tween-20 (PBST) for 1 h followed by incubation with 1:2000 diluted 6×-His tag mAb (produced in mouse; Invitrogen; #MA1-21315) at 4 °C, overnight.

    Incubation:

    Article Title: Endurance Exercise Enhances the Effect of Strength Training on Muscle Fiber Size and Protein Expression of Akt and mTOR
    Article Snippet: .. The gel was then incubated in transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% methanol) at 4°C for 30 min before electrophoretic transfer of the proteins to a polyvinylidine fluoride membrane (Bio-Rad, Laboratories, Richmond, CA) for 3 h on ice in a 4°C cold room. .. The membrane was subsequently blocked at room temperature for 1 h in TBS (Tris buffered saline; 10 mM Tris pH 7.6, 100 mM NaCl) containing 5% non-fat dry milk and then incubated overnight at 4°C with commercially available primary antibodies diluted in TBS containing 0.1% Tween 20 (TBST) with 2.5% non-fat dry milk: GAPDH (1:5,000), Akt (1:1,000), mTOR (1:1,000), S6K1 (1:1,000) purchased from Cell Signaling Technology (Beverly, MA); α-tubulin (1:5,000) from Sigma-Aldrich (St. Louis, MO); MAFbx (1:1,000) from Abcam (Cambridge, UK) and MuRF1 (1:1,000) from Santa Cruz Biotechnology (Santa Cruz, CA).

    Saline:

    Article Title: Overexpression of Diacylglycerol Kinase η Enhances G α q -Coupled G Protein–Coupled Receptor Signaling
    Article Snippet: A 15- μ l portion of each lysate used in the in vitro kinase assay was mixed with an appropriate amount of 4× Laemmli sample buffer—125 mM Tris-HCl, 40% (v/v) glycerol, 2% (v/v) SDS, 10% (v/v) β -mercaptoethanol (Bio-Rad, Hercules, CA), 0.02% (w/v) bromophenol blue—and separated by SDS-PAGE on a 4% to 15% gradient gel (Bio-Rad) at 100 V for 1 hour. .. Proteins were transferred to a polyvinylidine fluoride membrane (Bio-Rad) at 100 V for 70 minutes on ice and blocked with 5% nonfat milk (Bio-Rad) in Tris-buffered saline/Tween 20 (TBST; 100 mM Tris-HCl pH 7.5, 165 mM NaCl, 0.1% Tween 20) for 1 hour at room temperature. .. Blots were then incubated with rabbit anti-RFP primary antibody (Invitrogen) in 5% BSA (Sigma-Aldrich) in TBST overnight at 4°C.



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